Data Details
Disease Name liver cirrhosis
Tissue liver
Database BioProject
Data ID PRJNA553529
Data Type sequence
Platform GPL21493
Species mus musculus
Sample Number 2
PubMed ID 34277612
Title Single-cell analysis of whole hepatic non-parenchymal cells from normal and CCl4-injured mice
Data Description "Two male mice of 8-week old were injected i.p with olive oil and CCl4 independently twice a week for a total 6 weeks. Then, liver non-parenchymal cells were obtained by a two-step collagenase perfusion method. Hepatocytes were elimanated by three times of low-speed centrifugation at 50g for three minutes and were not included in further analysis of this study. Single-cell suspensions were then loaded on a Chromium Single Cell Instrument (10X Genomics) to generate single-cell GEMs. After reverse transcription, libraries were prepared using version 3 Chromium Single-cell 3' Library, Gel Bead & Mutiplex Kit (10X Genomics). Sequencing was performed on illumina HiSeq 3000."
Keyword cirrhosis single cell mrna
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