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| Data Details |
| Disease Name |
liver cirrhosis |
| Tissue |
liver |
| Database |
BioProject |
| Data ID |
PRJNA553529 |
| Data Type |
sequence |
| Platform |
GPL21493 |
| Species |
mus musculus |
| Sample Number |
2 |
| PubMed ID |
34277612 |
| Title |
Single-cell analysis of whole hepatic non-parenchymal cells from normal and CCl4-injured mice |
| Data Description |
"Two male mice of 8-week old were injected i.p with olive oil and CCl4 independently twice a week for a total 6 weeks. Then, liver non-parenchymal cells were obtained by a two-step collagenase perfusion method. Hepatocytes were elimanated by three times of low-speed centrifugation at 50g for three minutes and were not included in further analysis of this study. Single-cell suspensions were then loaded on a Chromium Single Cell Instrument (10X Genomics) to generate single-cell GEMs. After reverse transcription, libraries were prepared using version 3 Chromium Single-cell 3' Library, Gel Bead & Mutiplex Kit (10X Genomics). Sequencing was performed on illumina HiSeq 3000." |
| Keyword |
cirrhosis single cell mrna |
| Download |
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